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KMID : 0861020120270020061
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Korea Journal of Herbology
2012 Volume.27 No. 2 p.61 ~ p.67
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Neuroprotective effect of the water extract of Angelicae Gigantis Radix Palva in ischemic stroke rats
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Park Ki-Ho
Oh Tae-Woo
Park Yong-Ki
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Abstract
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Objective: Angelica Gigas Nakai is a popular oriental medicine used for the treatment of vascular diseases. The
aim of this study is to investigate neuroprotective effect of the water extract of Anelicae Gigantis Radix Palva
(AG) in transient middle cerebral artery occlusion (tMCAO)-induced ischemic rats via the regulation of
angiogenesis-related molecules.
Methods: Sprague-Dawley rats were intraperitoneally administrated with AG water extract at doses of 10, 25,
50 ㎎/㎏ body weight after tMCAO (90 min occlusion). reperfusion for 24 hr infarction volumes were measured
by 2,3,5-tetrazolium chloride (TTC) staining. Brain tissues were observed neuronal cell injuries by nissl
staining, and also brain-blood barrier (BBB) permeability change by evans blue. Expression of vascular
endothelial growth factor (VEGF), angiopoietin-1 (Ang-1) and Tie-2 receptor protein in brain tissues was
determined by western blot.
Results: AG water extract significantly reduced infarction volume in ischemic brains of rats, degradation of
neuronal cell, BBB permeability and expression of VEGF protein dose-dependently. Ang-1 protein was increased
dose-dependantly, not significantly.
Conclusion: This study suggests that AG water extract shows neuroprotective effect by preventing BBB
breakdown, with regulating angiogenesis factor VEGF and Ang-1.
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KEYWORD
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Angelica gigas, middle cerebral artery occlusion, brain-blood barrier, vascular endothelial growth factor, angiopoietin-1
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